Multiple Site Mutagenesis with High Targeting Efficiency in One Cloning Step
نویسندگان
چکیده
منابع مشابه
Multiple site mutagenesis with high targeting efficiency in one cloning step.
Site-directed mutagenesis is a widely used technique in molecular biology to generate single or multiple exchanges, insertions or deletions in DNA sequences. A number of strategies have been developed and used successfully (2,4). The QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) has proven valuable because it consistently yields high mutagenesis efficiencies and uses...
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BACKGROUND Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques. The ability to quickly and specifically mutate a residue(s) in protein is important for mechanistic and functional studies. Although many site-directed mutagenesis methods have been...
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BACKGROUND Various DNA manipulation methods have been developed to prepare mutant genes for protein engineering. However, development of more efficient and convenient method is still demanded. Homologous DNA assembly methods, which do not depend on restriction enzymes, have been used as convenient tools for cloning and have been applied to site-directed mutagenesis recently. This study describe...
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Mutagenesis is an important tool to study gene regulation, model disease-causing mutations and for functional characterisation of proteins. Most of the current methods for mutagenesis involve multiple step procedures. One of the most accurate methods for genetically altering DNA is recombineering, which uses bacteria expressing viral recombination proteins. Recently, the use of in vitro seamles...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00282bm01